Kinetic studies on the inhibition of Proteus vulgaris beta-lactamase by imipenem.
نویسندگان
چکیده
Imipenem was found to inhibit Proteus vulgaris beta-lactamase in a progressive manner. Kinetic experiments confirmed that the inactivated enzyme was not completely recovered after intact imipenem had been exhausted.
منابع مشابه
Prevalence of extended spectrum beta-lactamase production among uropathogens in south Mumbai and its antibiogram pattern
β-lactams are the most widely used group of antimicrobials. However, increasing resistance to these valuable drugs in uropathogens, mediated principally by β-lactamases, has become a major concern. The present study was undertaken to determine the prevalence of Extended Spectrum β-Lactamase (ESBL) producers in clinical isolates of urine specimens, collected from various healthcare centres acros...
متن کاملThe Journal of Antibiotics Ps-5 Inhibition of a B-lactamase from Proteus Vulgaris*
Inhibition of Proteus vulgaris B-lactamase by a new B-lactam antibiotic, PS-5 was studied kinetically. There were two stages of inhibition. In the early stage, PS-5 inhibited the Blactamase by formation of a MICHAELIS-complex, and showed a competitive inhibition pattern with Kj-value of 0.22 uM (substrate, cephaloridine). After the formation of a MICHAELIScomplex between PS-5 and the enzyme, PS...
متن کاملParadoxical antibacterial activities of beta-lactams against Proteus vulgaris: mechanism of the paradoxical effect.
Fifteen beta-lactam antibiotics were divided into four classes based on their antibacterial actions and beta-lactamase-inducing activities in Proteus vulgaris. One of these groups, which included cefmenoxime, ceftriaxone, cefuzonam, and cefotaxime, showed a clear paradoxical antibacterial activity against P. vulgaris. This group showed growth-inhibitory activity at relatively low concentrations...
متن کاملIsolation of TEM beta-lactamase gene in Pseudomonas aeruginosa and Imipenem Effect on Expression of TEM Gene by Real-Time PCR from Burn Wound Samples
Background & Aim: Pseudomonas aeruginosa strains that were resistance to majority of commonly used antibiotics were caused problem in treatment of these infections. Imipenem is the excessive potential antibiotic for elimination of antibiotic resistance isolates of these bacteria. Aim of this study was, identification of imipenem effect on TEM beta-lactamase gene expression in resistant to antib...
متن کاملSimple assay of beta-lactamase with agar medium containing a chromogenic cephalosporin, pyridinium-2-azo-p-dimethylaniline chromophore (PADAC).
A new beta-lactamase assay method with agar plates containing pyridinium-2-azo-p-dimethylaniline chromophore (PADAC) (50 microM), a beta-lactamase-labile, chromogenic cephalosporin, was examined. On the PADAC plates inoculated with beta-lactamase-producing gram-negative bacteria (10(4) CFU per spot) and incubated at 37 degrees C, a yellow zone showing hydrolysis of PADAC by beta-lactamase was f...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Antimicrobial agents and chemotherapy
دوره 25 1 شماره
صفحات -
تاریخ انتشار 1984